Agarose Gel Electrophoresis Weight/Volume (% w/v) Casting Calculator

Weigh agarose powder and SYBR/Ethidium stain for 0.8%–2.0% TAE/TBE gels.

Agarose Powder Weight Required
0.72 g
Agarose Weight in Milligrams 720 mg
1× DNA Stain Volume to Add 6 μL
1× Running Buffer in Casting Tray 60 mL
Inputs
% w/v
Target agarose gel concentration (% w/v, e.g., 1.0% or 1.5%).
mL
1× TAE or TBE casting tray volume in milliliters (mL).
×
Fluorescent DNA dye stock concentration (typically 10,000×).
Results & Graph
Agarose Powder Weight Required
0.72 g
Agarose Weight in Milligrams
720 mg
1× DNA Stain Volume to Add
6 μL
1× Running Buffer in Casting Tray
60 mL

Agarose Powder Weight Required

Graph 1 • Gel Concentration Pct Sensitivity Recalculates the domain equation across 50% to 150% of the primary input while holding other parameters constant.
0 g 0.6 g 1.2 g 50%: 0.36 g 0.4 50% 75%: 0.54 g 0.5 75% 100%: 0.72 g 0.7 100% 125%: 0.9 g 0.9 125% 150%: 1.08 g 1.1 150%
Graph 2 • Primary & Supporting Metric Comparison Compares the primary output alongside supporting domain benchmarks.
Agarose Powder Weight Required 0.72 g Agarose Weight in Milligrams 720 mg 1× DNA Stain Volume to Add 6 μL 1× Running Buffer in Casting Tray 60 mL
Graph 3 • Exact Output Table & Units Displays each calculated output in its exact domain unit.
Agarose Powder Weight Required0.72 gAgarose Weight in Milligrams720 mg1× DNA Stain Volume to Add6 μL1× Running Buffer in Casting Tray60 mL
Summary: To cast a 1.2% (w/v) gel in 60 mL buffer, microwave 0.72 g agarose (720 mg) and add 6 μL of 10000× DNA stain.
Summary: To cast a 1.2% (w/v) gel in 60 mL buffer, microwave 0.72 g agarose (720 mg) and add 6 μL of 10000× DNA stain.
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Interactive chart & math engine powered by CalculatorGraph

Formula & Mathematical Methodology: Agarose Gel Recipe Reference Equation

Agaroseg = (GelPercentage_% / 100) × BufferVolumemL
Stain_μL = BufferVolumemL / 10
Agarose_g = (GelPercentage_% / 100) × BufferVolume_mL; Stain_μL = BufferVolume_mL / 10
  • gelConcentrationPct: Gel Concentration Pct (% w/v)
  • bufferVolumeMl: Buffer Volume Ml (mL)
  • dnaStainStockFoldX: Dna Stain Stock Fold X (×)

Rounding: Laboratory and clinical equations retain full floating-point precision and round displayed results to 2 decimal places.

Worked Example: Reference Calculation: Agarose Gel Recipe

Weigh agarose powder and SYBR/Ethidium stain for 0.8%–2.0% TAE/TBE gels.

  1. Enter the reagent concentrations, assay readings, or clinical laboratory parameters.
  2. Compute the standard formula: Agarose_g = (GelPercentage_% / 100) × BufferVolume_mL; Stain_μL = BufferVolume_mL / 10.
  3. Review the primary result, supporting reference intervals, and 3 SVG visualizations.
Result: Evaluates Agarose Gel Recipe via Agarose_g = (GelPercentage_% / 100) × BufferVolume_mL; Stain_μL = BufferVolume_mL / 10.

Frequently Asked Questions

What formula is used by the Agarose Gel Electrophoresis Weight/Volume (% w/v) Casting Calculator?

It computes Agarose_g = (GelPercentage_% / 100) × BufferVolume_mL; Stain_μL = BufferVolume_mL / 10 and plots three custom SVG graphs showing parameter sensitivity and component breakdowns.

Can I compare different reagent dilutions or lab scenarios side by side?

Yes. Use the "Compare (0/3)" button to pin up to 3 scenarios side by side.

Are entered values stored on any server?

No. Every calculation runs 100% locally in your browser.

Science & Engineering · Molecular Biology & Genetics

Agarose Gel Electrophoresis Weight/Volume (% w/v) Casting Calculator: inputs, method, and how to read the result

Weigh agarose powder and SYBR/Ethidium stain for 0.8%–2.0% TAE/TBE gels.

Choose inputs for the case you are checking

The defaults are illustrative. Replace them with values for your case and keep the units shown beside each field.

  • Gel Concentration Pct: Target agarose gel concentration (% w/v, e.g., 1.0% or 1.5%).
  • Buffer Volume Ml: 1× TAE or TBE casting tray volume in milliliters (mL).
  • Dna Stain Stock Fold X: Fluorescent DNA dye stock concentration (typically 10,000×).

What the calculation includes

With the default values shown, Agarose Powder Weight Required is 0.72 g. The calculation follows Agarose Gel Recipe Reference Equation; the formula and variable definitions above show how the inputs combine.

One-input check: With the other defaults held constant, changing Gel Concentration Pct from 1.2 % w/v to 1.32 % w/v changes Agarose Powder Weight Required from 0.72 g to 0.79 g. This is a comparison inside the calculator's stated model, not a prediction.

Where to use caution

  • Uses standard IUPAC atomic weights, 25°C (298.15 K) reference thermodynamics, and published clinical literature equations.
  • Educational and laboratory reference tool only; clinical scoring calculators do not replace professional medical judgment or institutional protocols.

Scope: IUPAC / NIH Laboratory & Clinical Reference Standard Check the assumptions and limitations against your situation before relying on the estimate.

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